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Bioinformatics : Recent Questions and Answers (Page 44)

Find answers to your questions about Bioinformatics or help others by answering their Bioinformatics questions.

How to get rows with similar values in two different columns using command line?

I have following example data from a large file.cont100 1128 1125cont1005 3642 3642cont1006 103 19cont1037 3146 3146cont104 895 890cont1056 934 934cont1059 1750 1750cont1072 2577 2577cont1078 43 42cont1081 727 2I...

Asked on 11/28/2020 by MudithMMBc

2 answer

How to quantify similarity of genomes and find differences in set of S aureus genomes?

I have around 500 annotated proteomes of different bacterial strains and would like to quantify their similarity (or difference). I found gt genomediff from genometools gives me...

Asked on 11/26/2020

1 answer

Python equivalent to R GRanges and IRanges

Problem: I am trying to convert some codes written in R to Python and part of that conversion process is find classes equivalent to the GRanges and IRanges from the...

Asked on 11/26/2020

1 answer

Detecting Allelic Imbalance

I am interested in detecting allelic imbalance for a credible set of SNPs. Currently, I am looking for some packages, hopefully, for Python, that can assist with this. I am...

Asked on 11/26/2020 by Krizbomb

0 answer

Mapping statistics from bam file using bbtools and sambamba

Below are the statistics for RNA-seq mapped and unmapped paired-end reads to rice genome using reformat.sh from bbtools on bam files. It gives 77% mapped and 5% unmapped, what about...

Asked on 11/25/2020 by bioinfonext

2 answer

Call NCBI Multiple Sequence Alignment Viewer with target URL?

I am storing a sequence aligment file on a webserver and now would like to visualize the results. I used the NCBI Multiple Sequence Alignment Viewer ....

Asked on 11/24/2020

0 answer

annotation using ChIPseeker package error

I have differential binding sites object obtain from diffBind (dba.report). I am using the ChIP Seeker package to annotate the peaks but keep getting the following error: Error in (function...

Asked on 11/18/2020

0 answer

Preprocessing Affymetrix SNP Array Matrix

I'm working with public dataset from here: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE67047. I have downloaded the Series Matrix file, and I can extract metadata and genotype from it. I have some...

Asked on 11/17/2020 by Thanh Nguyen

0 answer

Get protein names corresponding to PDB ID

I have a list of about 4000 PDB IDs and would like to get the actual names of the proteins (e.g. lactate dehydrogenase, cytochrome c). I tried the batch header...

Asked on 11/13/2020

2 answer

Details of DESeq2 modeling a batch effect

When correcting my data for a batch effect using removeBatchEffect, some of the gene expression values become negative. When searching for differentially expressed genes, I do not use the data...

Asked on 11/09/2020

1 answer

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