What is 'k' in sequencing?

Question

When a DNA sequence is sequenced, I've only ever dealt with A,T,C,G and N which indicates un-identifiable bases. However, I came across a 'k' recently and I had asked another researcher who gave me an answer for what 'k' represents but I don't quite recall. It can't just be an anomaly in that one sequence file. Any ideas?

user6690 · Accepted Answer

See IUPAC codes:

So, as you can see above, K means "Either G or T".

Michael · Answer

It is recommended you learn the degenerate nucleotide code. In sequencing it can signify poor quality sequence data, but in primer design it is useful. R (mutation within purines) and Y (mutation within pyrimidines) are common. K, a purine to pyrimadine or pyrimadine to purine mutation is, in my opinion, rare. I would treat a K mutation with caution and consider the triplet codon around it, i.e. if it is part of a protein gene.

Most phylogeneitcs programs will work with the degenerate nucleotide code, so in theory you can still obtain useful information with it.

What is 'k' in sequencing?

2 Answers

Add your own answers!

Ask a Question