RuntimeError: cannot set daemon status of active thread

Contexto:

La clase “Main()es la encargada de ejecutar todas las funciones principales del programa, adicionalmente a esto la claseHilo()` se encarga de ejecutar una función en un hilo diferente.

Problema:

Al iniciar el programa la primera vez se ejecuta sin problema pero cuado el programa se vuelve a ejecutar lanza el siguiente error: raise RuntimeError("cannot set daemon status of active thread") RuntimeError: cannot set daemon status of active thread

Funcionamiento

Traceback

Traceback (most recent call last):
  File "D:Practicas PythonDetener HilosTestThreading.py", line 23, in <lambda>
    self.Next.clicked.connect(lambda:self.Fun1())
  File "D:Practicas PythonDetener HilosTestThreading.py", line 36, in Fun1
    self.Hilo.daemon = True
  File "C:UsersAngel-HpAppDataLocalProgramsPythonPython36libthreading.py", line 1141, in daemon
    raise RuntimeError("cannot set daemon status of active thread")
RuntimeError: cannot set daemon status of active thread
[Finished in 39.5s]

Codigo:

from PyQt5.QtWidgets import QMainWindow,QApplication
from PyQt5 import QtCore,QtGui
from PyQt5 import uic
import threading
import time

class Hilo(threading.Thread):
    def __init__(self,obj,fun):
        threading.Thread.__init__(self)

        self.fun = fun
        self.obj = obj

    def run(self):
        self.fun()

class Main(QMainWindow):
    signal = QtCore.pyqtSignal(object)
    def __init__(self):
        QMainWindow.__init__(self)
        uic.loadUi("Test.ui",self)

        self.Next.clicked.connect(lambda:self.Fun1())
        self.Back.clicked.connect(lambda:self.tabWidget.setCurrentIndex(0))
        self.signal.connect(self.Fun2)
        self.Hilo = Hilo(self,self.FunX)

    def Fun1(self):
        _movie = QtGui.QMovie("Loader.gif")
        self.label.setMovie(_movie)
        self.label.setScaledContents(True)
        _movie.start()



        self.Hilo.daemon = True
        self.Hilo.start()

    def FunX(self):
        print(threading.enumerate())
        time.sleep(5)
        self.signal.emit(0)

    def Fun2(self,valor):
        if valor == 0:
            self.label.setPixmap(QtGui.QPixmap("Confirmacion.png"))
            QtCore.QTimer.singleShot(2000,lambda:self.tabWidget.setCurrentIndex(1))
            self.Hilo._stop()
            print(threading.enumerate())

app = QApplication([])
m = Main()
m.show()
app.exec_()

class Main(QMainWindow):
    signal = QtCore.pyqtSignal(object)
    def __init__(self):
        QMainWindow.__init__(self)
        uic.loadUi("Test.ui",self)

        self.Next.clicked.connect(lambda:self.Fun1())
        self.Back.clicked.connect(lambda:self.tabWidget.setCurrentIndex(0))
        self.signal.connect(self.Fun2)
        self.Hilo = Hilo(self,self.FunX) #<-- Eliminar esta linea


    def Fun1(self):
        _movie = QtGui.QMovie("Loader.gif")
        self.label.setMovie(_movie)
        self.label.setScaledContents(True)
        _movie.start()


        self.Hilo = Hilo(self,self.FunX) #<-- Añadir esta linea
        self.Hilo.setDaemon(True)
        self.Hilo.start()